DNA degradation | Gel electrophoresis

There are several reasons why DNA can degrade over time. Some of the most common reasons include: Exposure to environmental factors: DNA can be degraded by exposure to environmental factors such as temperature, humidity, light, and oxygen. For example, exposure to high temperatures can cause the DNA strands to break apart, while exposure to ultraviolet (UV) light can cause chemical changes in the DNA that can break the bonds between nucleotides. Chemical damage: DNA can be damaged by exposure to chemicals such as acids, bases, and reactive oxygen species (ROS). These chemicals can alter the structure of the DNA molecule and cause the DNA strands to break apart. Enzymatic degradation: DNA can be degraded by enzymes that break down the DNA molecule. For example, DNases are enzymes that specifically target and break down DNA. Age: Over time, DNA can naturally degrade due to age-related changes. As the DNA molecule is exposed to various environmental factors and undergoes chemical changes, the bonds between the nucleotides can become weaker, leading to DNA degradation. Microbial activity: Microorganisms such as bacteria and fungi can degrade DNA through the action of their enzymes. Overall, DNA degradation can occur due to a variety of factors, and the rate of degradation can vary depending on the specific circumstances. It is important to store DNA samples properly to minimize degradation and preserve the quality of the DNA for future use. Problem: I just have been doing some extractions of DNA but currently, my electrophoresis has been like this. The first well is the molecular weight marker and the eighth well is another sample I ran some time ago and is ok. I use a new buffer, new free nuclease water, I cleaned my pipettes and so, but now that I put another sample in the eighth well as control I think my DNA is degraded but I don’t know. I have to say the voltage I used is 44 because the power supply doesn’t give more. #dna #gelelectrophoresis #genetics